human tslp elisa kit  (Bioss)

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: Differential expression of inflammation-related proteins between UST responders and non-responders.( A ) Volcano plot illustrating the differential expression of 92 inflammation-related proteins measured using the Olink ® platform. Red dots represent significantly upregulated proteins, blue dots indicate significantly downregulated proteins, and gray dots denote non-significant proteins. ( B ) Box-and-scatter plots showing the NPX of IL8, CD6, and TSLP in UST responders versus non-responders. ( C ) Heatmap depicting the expression profiles of the three DEPs across all participants. ( D ) ROC curve of IL8. ( E ) ROC curve of CD6. ( F ) ROC curve of TSLP.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Quantitative Proteomics, Expressing

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: External ELISA validation and diagnostic performance of DEPs.( A ) Validation of plasma protein levels in an independent cohort of responders (n = 10) and non-responders (n = 10) using ELISA. Box plots show the distribution of IL8, CD6, and TSLP concentrations between groups. ( B ) ROC curves evaluating the diagnostic value of each biomarker.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Diagnostic Assay, Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: Performance and visualization of the logistic regression model for predicting response to UST.( A ) Variable importance plot of the multivariate logistic regression model based on three differentially expressed proteins (IL8, CD6, and TSLP).( B ) ROC curve of the model in the original dataset.( C ) Nomogram constructed based on the logistic regression model to facilitate individualized prediction of UST response.( D ) Calibration curve generated using 500 bootstrap resamples, assessing agreement between predicted and observed probabilities.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Construct, Generated

Journal: International Journal of Molecular Sciences

Article Title: Protective Effect of Camellia japonica Extract on 2,4-Dinitrochlorobenzene (DNCB)-Induced Atopic Dermatitis in an SKH-1 Mouse Model

doi: 10.3390/ijms26157286

Figure Lengend Snippet: Effects of Camellia japonica extract on ( a ) IgE, ( b ) histamine, and ( c ) TSLP levels in mice with DNCB-induced AD. Data are presented as the mean ± standard deviation (SD); analysis of variance (ANOVA) and Tukey’s test. * p < 0.05, *** p < 0.001 vs. NC. NT, no treatment group; NC, negative control group; PC, positive control group; LT, low-concentration 4% extract treatment group; MT, mid-concentration 20% extract treatment group; HT, high-concentration 100% extract treatment group.

Article Snippet: Serum total TSLP concentrations were measured using a TSLP ELISA kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Standard Deviation, Negative Control, Positive Control, Concentration Assay